Human ATP5H Recombinant Protein (RPPB2824)

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SKU:
RPPB2824
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
ATP5H
Synonyms:
ATP Synthase H+ Transporting
Synonyms:
Mitochondrial Fo Complex Subunit D
Synonyms:
ATP Synthase D Chain Mitochondrial
Synonyms:
ATP Synthase H+ Transporting Mitochondrial F1F0 Subunit D
Source:
Escherichia Coli
Uniprot:
O75947
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Description

Product Name:Human ATP5H Recombinant Protein
Product Code:RPPB2824
Size:10µg
Species:Human
Target:ATP5H
Synonyms:ATP Synthase H+ Transporting, Mitochondrial Fo Complex Subunit D, ATP Synthase D Chain Mitochondrial, ATP Synthase H+ Transporting Mitochondrial F1F0 Subunit D, ATPase Subunit D, My032 Protein, ATPQ.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The ATP5H solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGRKLA LKTIDWVAFA EIIPQNQKAI ASSLKSWNET LTSRLAALPE NPPAIDWAYY KANVAKAGLV DDFEKKFNAL KVPVPEDKYT AQVDAEEKED VKSCAEWVSL SKARIVEYEK EMEKMKNLIP FDQMTIEDLN EAFPETKLDK KKYPYWPHQP IENL

ATP5H is a member of the ATPase d subunit family and encodes the d subunit of the F0 complex. Electron transport complexes of the respiratory chain create a proton gradient across the membrane which induces ATP5H to transform ADP to ATP. Two alternatively spliced isoforms of ATP5H, encoded by a gene that maps to human chromosome 17q25.1, are known. ATP5H is restricted to mitochondrial inner membrane.

ATP5H Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids (1-161) and having a molecular mass of 20.9kDa.ATP5H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ATP5H: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase d subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function:ATPase activity; transmembrane transporter activity; hydrogen ion transmembrane transporter activity

Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport

NCBI Summary:Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]
UniProt Code:O75947
NCBI GenInfo Identifier:6831494
NCBI Gene ID:10476
NCBI Accession:O75947.3
UniProt Secondary Accession:O75947,Q9H3J4, B2R5L6,
UniProt Related Accession:O75947
Molecular Weight:Observed MW 18kDaCalculated MW 15kDa/18kDa
NCBI Full Name:ATP synthase subunit d, mitochondrial
NCBI Synonym Full Names:ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
NCBI Official Symbol:ATP5H  
NCBI Official Synonym Symbols:ATPQ  
NCBI Protein Information:ATP synthase subunit d, mitochondrial; My032 protein; ATPase subunit d; ATP synthase D chain, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1F0, subunit d; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
UniProt Protein Name:ATP synthase subunit d, mitochondrial
UniProt Gene Name:ATP5H  
UniProt Entry Name:ATP5H_HUMAN
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