Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

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SKU:
HUDL00030
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UNQ0
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CDw338,CD338,ABC-G2,MRX,BCRP1,ABC15,ABCP,BCRP,BMDP,MXR,MXR1,Mitoxantrone resistance-associated protein,Breast Cancer Resistance Protein 1,Urate exporter
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

The Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit is specifically designed for the precise quantification of ABCG2 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.ABCG2 is a vital protein involved in drug transport and resistance, playing a crucial role in determining the efficacy of cancer chemotherapy treatments.

Its expression is also linked to various other diseases, including cardiovascular disorders and neurodegenerative conditions. As a key biomarker for studying drug resistance mechanisms and developing new therapeutic strategies, the Human ABCG2 ELISA Kit is a valuable tool for researchers in the fields of oncology, pharmacology, and drug development.

Product Name:Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit
Product Code:HUDL00030
Size:96 Assays
Target:ABCG2
Synonyms:CDw338, CD338, ABC-G2, MRX, BCRP1, ABC15, ABCP, BCRP, BMDP, MXR, MXR1, Mitoxantrone resistance-associated protein, Breast Cancer Resistance Protein 1, Urate exporter
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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