Human ATP Binding Cassette Transporter G1 (ABCG1) ELISA Kit
The Human ATP Binding Cassette Transporter G1 (ABCG1) ELISA Kit is a powerful tool for researchers studying lipid metabolism and cardiovascular diseases. This kit is specifically designed for the accurate quantification of ABCG1 levels in human serum, plasma, and cell culture supernatants.ABCG1 is a key transporter protein that plays a critical role in cholesterol homeostasis and lipid metabolism. Dysregulation of ABCG1 has been implicated in various cardiovascular diseases, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.
With its high sensitivity and specificity, this ELISA kit provides reliable and reproducible results, making it suitable for a wide range of research applications. By measuring ABCG1 levels, researchers can gain valuable insights into lipid transport and metabolism, paving the way for new treatment strategies for cardiovascular diseases.
Product Name:
Human ATP Binding Cassette Transporter G1 (ABCG1) ELISA Kit
Product Code:
HUDL00029
Size:
96 Assays
Target:
ABCG1
Synonyms:
ABC-G1, ABC8, WHITE1, WHT1, ATP-Binding Cassette Transporter 8, White protein homolog
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
