Human ATM (Ataxia Telangiectasia Mutated) CLIA Kit
The Human ATM (Ataxia Telangiectasia Mutated) CLIA Kit is specifically designed for the quantitative measurement of ATM levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and precision, allowing for reliable and consistent results across various research applications.ATM is a key protein involved in DNA damage response and repair pathways, playing a crucial role in maintaining genomic stability. Mutations in the ATM gene are linked to the development of Ataxia Telangiectasia, a rare genetic disorder characterized by neurological abnormalities and an increased risk of cancer.
By measuring ATM levels, researchers can gain valuable insights into the pathophysiology of Ataxia Telangiectasia and potentially identify novel therapeutic targets for the condition.Overall, the Human ATM CLIA Kit provides researchers with a powerful tool for studying ATM biology and its implications in disease, offering a valuable resource for advancing our understanding of DNA repair mechanisms and potential treatment strategies.
Product Name:
Human ATM (Ataxia Telangiectasia Mutated) CLIA Kit
SKU:
HUES00368
Target:
Human ATM (Ataxia Telangiectasia Mutated)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ATM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ATM. You can calculate the concentration of Human ATM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
85-98
86-97
Average (%)
98
90
92
1:4
Range (%)
86-98
92-109
88-101
Average (%)
93
99
94
1:8
Range (%)
90-105
95-106
99-112
Average (%)
96
101
106
1:16
Range (%)
97-112
103-117
99-119
Average (%)
102
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
98-115
105
Cell culture media (n=5)
99-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.18
314.92
824.23
98.99
287.34
828.83
Standard deviation
12.26
29.13
64.04
9.29
33.65
86.36
C V (%)
12.75
9.25
7.77
9.38
11.71
10.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ATM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ATM in samples. No significant cross-reactivity or interference between Human ATM and analogues was observed.
