Human ATF6 (Activating Transcription Factor 6) ELISA Kit
The Human ATF6 (Activating Transcription Factor 6) ELISA Kit is specifically designed for the accurate detection of ATF6 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ATF6 is a key transcription factor that plays a critical role in the unfolded protein response pathway, regulating the expression of genes involved in protein folding and quality control.
Dysregulation of ATF6 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human ATF6 ELISA Kit provides a valuable tool for researchers to accurately measure ATF6 levels in biological samples, aiding in the understanding of its function and potential implications in disease pathogenesis.
Product Name:
Human ATF6 (Activating Transcription Factor 6) ELISA Kit
SKU:
HUES01722
Target:
Human ATF6 (Activating Transcription Factor 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATF6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATF6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ATF6. You can calculate the concentration of Human ATF6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
97-109
89-104
Average (%)
104
102
96
1:4
Range (%)
90-105
85-95
81-95
Average (%)
98
90
88
1:8
Range (%)
88-102
83-97
87-98
Average (%)
94
90
92
1:16
Range (%)
90-105
86-99
87-102
Average (%)
96
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.11
2.35
9.88
1.08
2.36
9.9
Standard deviation
0.07
0.1
0.48
0.06
0.1
0.48
C V (%)
6.31
4.26
4.86
5.56
4.24
4.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ATF6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ATF6 in samples. No significant cross-reactivity or interference between Human ATF6 and analogues was observed.
