The Human Antithrombin (AT) ELISA Kit is specifically designed for the precise measurement of antithrombin levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Antithrombin is a key protein that plays a critical role in regulating blood clot formation by inhibiting certain clotting factors.
Dysregulation of antithrombin levels has been linked to various thrombotic disorders, making it a valuable biomarker for studying and developing treatments for these conditions.With its reliable performance and ease of use, the Human Antithrombin ELISA Kit is an essential tool for researchers investigating the role of antithrombin in thrombosis, hemostasis, and related diseases.
Product Name:
Human AT (AntiThrombin) ELISA Kit
SKU:
HUES01644
Target:
Human AT (AntiThrombin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AT-Ⅲ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AT-Ⅲ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AT-Ⅲ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AT-Ⅲ. You can calculate the concentration of Human AT-Ⅲ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
88-99
87-98
Average (%)
103
94
92
1:4
Range (%)
91-107
81-93
89-101
Average (%)
98
85
94
1:8
Range (%)
91-108
82-97
82-94
Average (%)
99
88
88
1:16
Range (%)
93-106
86-100
85-99
Average (%)
100
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.17
269.09
783.72
97.0
262.22
780.69
Standard deviation
5.62
14.29
25.0
5.65
11.35
27.79
C V (%)
5.61
5.31
3.19
5.82
4.33
3.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AT-Ⅲ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AT-Ⅲ in samples. No significant cross-reactivity or interference between Human AT-Ⅲ and analogues was observed.
