Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.
Product Name:
Human Arginase/ARG1 Recombinant Protein (RPES4663)
Product Code:
RPES4663
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Accession:
NP_000036.2
Sequence:
Met 1-Lys322
Fusion tag:
C-6His
Activity:
Measured by the production of urea during the hydrolysis of arginine. The specific activity is 46411 pmol/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Arginase-1 is produced by our Mammalian expression system and the target gene encoding Met1-Lys322 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
35.6 kDa
AP Mol Mass:
38 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris, 150mMNaCl, 20%Glycerol,1mMDTT,pH7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
