Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.
Product Name:
Human Arginase/ARG1 Recombinant Protein (RPES0423)
Product Code:
RPES0423
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Accession:
NP_000036.2
Sequence:
Met 1-Lys 322
Fusion tag:
N-His&C-MYC
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human ARG1 (NP_000036.2) (Met1-Lys322) was expressed with a polyhistidine tag at the N-terminus and a myc tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
36.7 kDa
Formulation:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
