The Human Aquaporin 8 (AQP8) ELISA Kit is a cutting-edge tool for the precise measurement of AQP8 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees accurate and consistent results, making it an invaluable resource for a variety of research studies.Aquaporin 8 is a vital protein involved in the regulation of water balance in cell membranes, playing a crucial role in various physiological processes such as fluid transport and cell hydration. Dysregulation of AQP8 has been linked to conditions including liver diseases, kidney disorders, and inflammatory bowel disease, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
By utilizing the Human Aquaporin 8 ELISA Kit, researchers can gain deeper insights into the role of AQP8 in health and disease, paving the way for innovative advancements in medical research and personalized treatment strategies. Trust in this advanced kit to unlock the mysteries of AQP8 biology and drive new discoveries in biomedical science.
Product Name:
Human Aquaporin 8 (AQP8) ELISA Kit
Product Code:
HUDL00233
Size:
96 Assays
Target:
AQP8
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
