The Human AQP-2 (Aquaporin-2) CLIA Kit is a powerful tool for the precise measurement of aquaporin-2 levels in human biological samples. Aquaporin-2 is a water channel protein that plays a crucial role in water reabsorption in the kidneys, making it essential for maintaining water balance in the body.This CLIA kit offers high sensitivity and specificity, ensuring accurate and reliable results for researchers studying conditions related to water balance, such as kidney diseases, diabetes insipidus, and hypertension.
Its ease of use and quick turnaround time make it suitable for a wide range of research applications, providing valuable insights into the role of aquaporin-2 in health and disease.Invest in the Human AQP-2 CLIA Kit to uncover the mechanisms behind water balance regulation and discover potential therapeutic targets for conditions related to impaired kidney function and fluid imbalance.
Product Name:
Human AQP-2 (Aquaporin 2) CLIA Kit
SKU:
HUES00350
Target:
Human AQP-2 (Aquaporin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AQP-2. You can calculate the concentration of Human AQP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
103-115
89-103
Average (%)
99
109
94
1:4
Range (%)
96-112
89-101
92-106
Average (%)
104
94
98
1:8
Range (%)
100-117
101-114
89-102
Average (%)
107
107
96
1:16
Range (%)
100-114
87-103
93-106
Average (%)
106
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
232.61
788.63
2162.82
243.54
864.61
2189.91
Standard deviation
22.91
92.66
152.7
30.86
92.95
211.76
C V (%)
9.85
11.75
7.06
12.67
10.75
9.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AQP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AQP-2 in samples. No significant cross-reactivity or interference between Human AQP-2 and analogues was observed.
