The Human AQP-1 (Aquaporin 1) ELISA Kit is specifically designed for the quantitative detection of Aquaporin 1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and accurate, providing researchers with reliable and reproducible results for a variety of research applications.Aquaporin 1 is a key membrane protein that plays a crucial role in the regulation of water transport across cell membranes. It is involved in various physiological processes, including urine concentration, fluid balance, and cell migration.
Dysregulation of Aquaporin 1 has been linked to a range of disorders, such as kidney diseases, brain edema, and certain types of cancer, making it a valuable biomarker for studying these conditions and developing potential treatment strategies.Overall, the Human AQP-1 ELISA Kit offers researchers a powerful tool for investigating the role of Aquaporin 1 in health and disease, providing valuable insights into potential therapeutic targets and personalized medicine approaches.
Product Name:
Human AQP-1 (Aquaporin 1) ELISA Kit
SKU:
HUES01691
Target:
Human AQP-1 (Aquaporin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AQP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AQP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AQP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AQP-1. You can calculate the concentration of Human AQP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
95-106
87-101
Average (%)
95
101
93
1:4
Range (%)
86-98
83-98
82-94
Average (%)
93
90
89
1:8
Range (%)
91-103
81-91
89-102
Average (%)
96
86
95
1:16
Range (%)
93-109
83-96
81-95
Average (%)
100
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
399.9
1101.92
3738.97
437.93
1190.85
3925.0
Standard deviation
20.03
59.28
118.15
26.54
49.18
174.27
C V (%)
5.01
5.38
3.16
6.06
4.13
4.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AQP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed.
