The Human APOC3 (Apolipoprotein C3) ELISA Kit is specifically designed for the accurate quantitative measurement of apolipoprotein C3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Apolipoprotein C3 is a key protein involved in lipid metabolism, playing a crucial role in regulating triglyceride levels in the blood. Dysregulation of APOC3 has been linked to various metabolic disorders, such as hypertriglyceridemia and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With the Human APOC3 ELISA Kit, researchers can accurately quantify APOC3 levels in biological samples, furthering our understanding of lipid metabolism and metabolic diseases. This kit provides a reliable tool for investigating the role of APOC3 in health and disease, offering valuable insights for future research and therapeutic development.
Product Name:
Human ApoC3 (Apolipoprotein C3) ELISA Kit
SKU:
HUES01672
Target:
Human ApoC3 (Apolipoprotein C3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoC3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoC3. You can calculate the concentration of Human ApoC3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
98-117
96-109
Average (%)
105
106
104
1:4
Range (%)
98-116
82-95
98-111
Average (%)
106
87
104
1:8
Range (%)
99-114
86-100
92-106
Average (%)
106
93
99
1:16
Range (%)
95-109
85-96
97-112
Average (%)
102
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.6
65.8
176.8
25.7
62.1
170.4
Standard deviation
1.8
3.6
6.2
1.6
2.7
5.5
C V (%)
6.77
5.47
3.51
6.23
4.35
3.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoC3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoC3 in samples. No significant cross-reactivity or interference between Human ApoC3 and analogues was observed.
