The Human APOA4 (Apolipoprotein A4) ELISA Kit is specifically designed for the precise detection of apolipoprotein A4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Apolipoprotein A4 is a key protein involved in lipid metabolism and plays a crucial role in regulating cholesterol levels and lipid transport in the body. Abnormal levels of APOA4 have been associated with various metabolic disorders, including cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.
Overall, the Human APOA4 ELISA Kit provides researchers with a powerful tool for investigating the role of apolipoprotein A4 in health and disease, offering reliable and accurate measurements for a deeper understanding of its implications in metabolic disorders.
Product Name:
Human ApoA4 (Apolipoprotein A4) ELISA Kit
SKU:
HUES01668
Target:
Human ApoA4 (Apolipoprotein A4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
2.81 ng/mL
Detection range:
4.69-300 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA4. You can calculate the concentration of Human ApoA4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
89-105
90-105
Average (%)
101
97
97
1:4
Range (%)
97-111
84-96
96-110
Average (%)
104
88
102
1:8
Range (%)
94-109
84-97
92-103
Average (%)
101
90
97
1:16
Range (%)
92-110
85-98
94-107
Average (%)
100
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
14
45.8
115.8
12.7
47.4
118.0
Standard deviation
0.9
2.5
3.9
0.8
2.5
5.8
C V (%)
6.43
5.46
3.37
6.3
5.27
4.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoA4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.
