The Human APOA1 (Apolipoprotein A1) ELISA Kit is specifically designed for the accurate measurement of apolipoprotein A1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for various research purposes.Apolipoprotein A1 is a vital component of high-density lipoprotein (HDL) particles and plays a crucial role in lipid metabolism and cardiovascular health.
Abnormal levels of APOA1 have been linked to various cardiovascular diseases, making it an important biomarker for studying and diagnosing these conditions.With its easy-to-use format and reliable performance, the Human APOA1 ELISA Kit is an invaluable tool for researchers and clinicians looking to investigate the role of apolipoprotein A1 in health and disease.
Product Name:
Human ApoA1 (Apolipoprotein A1) ELISA Kit
SKU:
HUES01407
Target:
Human ApoA1 (Apolipoprotein A1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA1. You can calculate the concentration of Human ApoA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
97-109
95-108
Average (%)
97
103
101
1:4
Range (%)
99-112
81-94
93-103
Average (%)
107
87
98
1:8
Range (%)
102-113
84-99
97-110
Average (%)
107
90
103
1:16
Range (%)
98-113
84-95
96-111
Average (%)
105
90
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.3
17.3
76.1
8.9
15.7
83.2
Standard deviation
0.5
1.0
2.7
0.6
0.9
3.8
C V (%)
5.38
5.78
3.55
6.74
5.73
4.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA1 in samples. No significant cross-reactivity or interference between Human ApoA1 and analogues was observed.
