The Human APOA1 (Apolipoprotein A1) CLIA Kit is a highly accurate and reliable tool for measuring APOA1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.APOA1 is a pivotal protein known for its role in lipid metabolism and cardiovascular health. It is a key component of high-density lipoprotein (HDL) particles, also known as "good" cholesterol, and is involved in the transport of cholesterol from peripheral tissues to the liver for excretion.
Dysregulation of APOA1 has been linked to various cardiovascular diseases, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.Overall, the Human APOA1 CLIA Kit provides researchers with a powerful tool for accurately measuring APOA1 levels, facilitating further investigations into its biological functions and potential clinical applications.
Product Name:
Human ApoA1 (Apolipoprotein A1) CLIA Kit
SKU:
HUES00116
Target:
Human ApoA1 (Apolipoprotein A1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoA1. You can calculate the concentration of Human ApoA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
102-116
94-108
Average (%)
105
108
101
1:4
Range (%)
100-113
99-112
88-104
Average (%)
106
105
95
1:8
Range (%)
89-103
86-98
91-106
Average (%)
95
93
98
1:16
Range (%)
97-110
101-115
100-116
Average (%)
105
107
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
84-96
90
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.29
5.31
18.86
2.21
5.18
17.11
Standard deviation
0.28
0.63
1.32
0.28
0.41
1.71
C V (%)
12.23
11.86
7.0
12.67
7.92
9.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA1 in samples. No significant cross-reactivity or interference between Human ApoA1 and analogues was observed.
