The Human ANXA2 (Annexin A2) ELISA Kit is specifically designed for the precise measurement of annexin A2 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it well-suited for a wide array of research applications.Annexin A2 is a critical protein involved in various cellular processes, such as membrane trafficking, inflammation, and cell adhesion.
Its dysregulation has been linked to diseases like cancer, autoimmune disorders, and cardiovascular diseases, highlighting its importance as a biomarker for understanding these conditions and potential therapeutic interventions.Overall, the Human ANXA2 (Annexin A2) ELISA Kit is a valuable tool for researchers seeking to analyze annexin A2 levels and explore its role in health and disease.
Product Name:
Human ANXA2 (Annexin A2) ELISA Kit
SKU:
HUES01659
Target:
Human ANXA2 (Annexin A2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANXA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANXA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANXA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANXA2. You can calculate the concentration of Human ANXA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
89-105
93-106
Average (%)
102
97
100
1:4
Range (%)
93-109
82-95
82-93
Average (%)
100
88
88
1:8
Range (%)
92-103
86-96
88-99
Average (%)
97
91
93
1:16
Range (%)
91-103
85-100
81-91
Average (%)
98
92
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.02
3.69
18.13
1.96
3.63
18.51
Standard deviation
0.14
0.2
0.94
0.14
0.16
0.78
C V (%)
6.93
5.42
5.18
7.14
4.41
4.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANXA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANXA2 in samples. No significant cross-reactivity or interference between Human ANXA2 and analogues was observed.
