The Human Anion Sugar Transporter (AST) ELISA Kit is a specialized assay designed for the precise measurement of AST levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.AST is a critical transporter protein responsible for the movement of anion sugars across cell membranes, playing a vital role in cellular metabolism and overall physiological functions.
Abnormalities in AST expression have been linked to various health conditions, making it a valuable biomarker for research in diseases such as diabetes, obesity, and metabolic syndromes.By utilizing the Human AST ELISA Kit, researchers can gain valuable insights into the role of AST in health and disease, facilitating the development of novel therapeutic strategies and diagnostic tools. Trust in this advanced ELISA kit for robust and reliable data in your study of anion sugar transporter biology.
Product Name:
Human Anion/Sugar Transporter (AST) ELISA Kit
Product Code:
HUDL01560
Size:
96 Assays
Target:
AST
Synonyms:
SLC17A5, SD, ISSD, NSD, SIALIN, SIASD, SLD, Solute Carrier Family 17 Member 5, Sialic Acid Storage Disease, Vesicular H(+)/Aspartate-glutamate cotransporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
