Human ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit
The Human ANGPTL8 (Angiopoietin-like protein 8) ELISA Kit is specifically designed for the accurate and reliable detection of ANGPTL8 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ANGPTL8 is a critical protein that plays a key role in regulating lipid metabolism and insulin sensitivity.
It is involved in various metabolic processes, making it a valuable biomarker for studying metabolic disorders such as obesity, diabetes, and cardiovascular diseases. This ELISA kit provides researchers with a powerful tool to investigate the role of ANGPTL8 in disease development and progression, ultimately leading to potential therapeutic advancements in the field of metabolic research.
Product Name:
Human ANGPTL8 (Angiopoietin Like Protein 8) ELISA Kit
SKU:
HUES03101
Target:
Human ANGPTL8 (Angiopoietin Like Protein 8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANGPTL8. You can calculate the concentration of Human ANGPTL8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
89-101
95-112
Average (%)
105
96
103
1:4
Range (%)
88-103
84-97
89-99
Average (%)
94
89
94
1:8
Range (%)
93-107
82-97
86-96
Average (%)
99
88
91
1:16
Range (%)
94-108
80-91
86-101
Average (%)
100
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
93-108
98
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
388.23
875.98
2939.63
386.89
896.99
3163.19
Standard deviation
25.08
44.67
100.54
26.0
40.45
114.82
C V (%)
6.46
5.1
3.42
6.72
4.51
3.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANGPTL8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL8 in samples. No significant cross-reactivity or interference between Human ANGPTL8 and analogues was observed.
