Human Angiotensin I Converting Enzyme (ACE) ELISA Kit

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SKU:
HUDL00036
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12821
Sensitivity:
0.18ng/mL
Range:
0.39-25ng/mL
ELISA Type:
Sandwich
Synonyms:
CD143,ACE1,DCP1,ACEI,ACE-I,Kininase II,Angiotensin-Converting Enzyme,Peptidyl-Dipeptidase A,Dipeptidyl Carboxypeptidase 1,Angiotensin-converting enzyme, soluble form
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Angiotensin I Converting Enzyme (ACE) ELISA Kit

The Human Angiotensin I-Converting Enzyme (ACE) ELISA Kit is specifically designed for the precise measurement of ACE levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it suitable for a variety of research applications.ACE is an important enzyme involved in the renin-angiotensin system, playing a key role in regulating blood pressure and fluid balance.

Dysregulation of ACE activity has been implicated in various cardiovascular diseases, making it a valuable biomarker for studying and understanding these conditions.By using the Human ACE ELISA Kit, researchers can accurately assess ACE levels in biological samples, advancing their understanding of the enzyme's role in health and disease. Order your kit now from AssayGenie for high-quality results and reliable data in your research endeavors.

Product Name:Human Angiotensin I Converting Enzyme (ACE) ELISA Kit
Product Code:HUDL00036
Size:96 Assays
Target:ACE
Synonyms:CD143, ACE1, DCP1, ACEI, ACE-I, Kininase II, Angiotensin-Converting Enzyme, Peptidyl-Dipeptidase A, Dipeptidyl Carboxypeptidase 1, Angiotensin-converting enzyme, soluble form
Detection Method:Sandwich
Range:0.39-25ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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