Human Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
The Human Angiotensin I-Converting Enzyme 2 (ACE2) ELISA Kit is a comprehensive tool for the precise measurement of ACE2 levels in human samples, including serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers consistent and dependable results, making it an invaluable asset for various research endeavors.ACE2 is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and cardiovascular function. Additionally, ACE2 serves as the cellular entry receptor for the SARS-CoV-2 virus, highlighting its significance in infectious diseases.
By accurately quantifying ACE2 levels, researchers can better understand its role in various physiological and pathological processes, facilitating the development of targeted therapies and interventions.The Human ACE2 ELISA Kit offers a user-friendly and efficient solution for studying ACE2 expression and activity, making it an essential tool for investigations into cardiovascular health, viral infections, and other disease states. With its high performance and versatility, this kit is an essential resource for advancing scientific knowledge and exploring novel treatment strategies.
Product Name:
Human Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
