Human Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit

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SKU:
HUDL00175
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9BY76
Sensitivity:
0.31ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
ARP4,FIAF,HFARP,NL2,PGAR,Pp1158,Fasting-Induced Adipose Factor,Hepatic Angiopoietin-Related Protein,Hepatic Fibrinogen/Angiopoietin-Related Protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit

The Human Angiopoietin-like Protein 4 (ANGPTL4) ELISA Kit is a cutting-edge tool for the precise measurement of ANGPTL4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring the generation of accurate and consistent data for a variety of research applications.ANGPTL4 is a key player in metabolic regulation, lipid metabolism, and cardiovascular health.

Its dysregulation has been linked to obesity, diabetes, and atherosclerosis, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.With its advanced technology and reliable performance, the Human ANGPTL4 ELISA Kit is a must-have for researchers seeking to delve deeper into the intricate mechanisms of metabolic disorders and cardiovascular diseases.

Product Name:Human Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit
Product Code:HUDL00175
Size:96 Assays
Target:ANGPTL4
Synonyms:ARP4, FIAF, HFARP, NL2, PGAR, Pp1158, Fasting-Induced Adipose Factor, Hepatic Angiopoietin-Related Protein, Hepatic Fibrinogen/Angiopoietin-Related Protein
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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