The Human ANG (Angiotensin II) ELISA Kit is a valuable tool for researchers conducting studies on angiogenin levels in human samples. This kit offers high accuracy and precision, enabling the detection of Angiotensin II in serum, plasma, and cell culture supernatants.Angiotensin II is a key player in angiogenesis and cell proliferation, impacting a variety of health conditions including cancer, cardiovascular diseases, and neurodegenerative disorders.
By accurately measuring Angiotensin II levels, researchers can gain insight into these conditions and potentially develop new therapeutic strategies.With its advanced technology and reliable performance, the Human ANG (Angiotensin II) ELISA Kit is an essential resource for researchers seeking to further explore the role of Angiotensin II in human health and disease.
Product Name:
Human Ang-II (Angiotensin II) ELISA Kit
SKU:
HUES01547
Target:
Human Ang-II (Angiotensin II)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
87-102
92-105
Average (%)
105
93
97
1:4
Range (%)
82-97
87-100
99-117
Average (%)
89
94
107
1:8
Range (%)
89-103
86-98
92-110
Average (%)
95
92
100
1:16
Range (%)
88-99
90-100
92-108
Average (%)
93
95
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.1
272.5
948.9
108.4
291.7
898.0
Standard deviation
6.7
14.4
43.6
6.6
14.6
44.0
C V (%)
6.63
5.28
4.59
6.09
5.01
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang-Ⅱ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
