Human Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit

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SKU:
HUDL00129
Product Type:
ELISA Kit
Sensitivity:
0.131ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ALAS3,ALAS,ALASH,5-aminolevulinic acid synthase 1,Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Human Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit

The Human Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit is specifically designed for the precise measurement of ALAS1 levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results, making it an ideal tool for various research purposes.ALAS1 is an important enzyme involved in heme biosynthesis and has been implicated in various diseases such as porphyrias and lead poisoning.

By measuring ALAS1 levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies.This ELISA kit offers a reliable and efficient method for quantifying ALAS1 levels, enabling researchers to further elucidate its role in disease pathology and explore its potential as a biomarker for diagnosis and treatment monitoring.

Product Name:Human Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit
Product Code:HUDL00129
Size:96 Assays
Target:ALAS1
Synonyms:ALAS3, ALAS, ALASH, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, 5-aminolevulinate synthase, nonspecific, mitochondrial
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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