Human Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit
The Human Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit is a powerful tool for the precise measurement of ALAD levels in human samples such as serum, plasma, and cell culture supernatants. This kit has been meticulously designed to provide high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ALAD is an enzyme critical for heme biosynthesis, playing a crucial role in various physiological processes including oxygen transport and metabolism. Dysregulation of ALAD has been linked to conditions such as lead poisoning, acute porphyria, and other metabolic disorders, making it a valuable biomarker for studying these diseases and exploring potential treatment options.
By utilizing the Human ALAD ELISA Kit, researchers can gain valuable insights into the role of ALAD in health and disease, leading to advancements in the diagnosis, prognosis, and therapeutic interventions for a range of medical conditions. Trust in the reliability and accuracy of this ELISA kit to enhance your research and contribute to scientific advancements in the field.
Product Name:
Human Aminolevulinate Delta Dehydratase (ALAD) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
