Human Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) ELISA Kit
The Human AIMP1 (Aminoacyl-tRNA Synthetase Complex-Interacting Multifunctional Protein 1) ELISA Kit is specifically designed for the accurate and sensitive detection of AIMP1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring reliable results for a variety of research applications.AIMP1 is a multifunctional protein that interacts with the aminoacyl-tRNA synthetase complex, playing a crucial role in protein synthesis and cellular functions.
Dysregulation of AIMP1 has been implicated in various diseases including cancer, inflammatory disorders, and autoimmune diseases. Therefore, this ELISA kit provides a valuable tool for researchers to study the role of AIMP1 in these pathologies and explore its potential as a therapeutic target.Overall, the Human AIMP1 ELISA Kit offers researchers a reliable and efficient method for quantifying AIMP1 levels in human samples, facilitating in-depth investigations into its biological functions and clinical significance.
Product Name:
Human Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) ELISA Kit
Product Code:
HUDL00124
Size:
96 Assays
Target:
AIMP1
Synonyms:
SCYE1, EMAP II, EMAP2, p43, Small Inducible Cytokine Subfamily E,Member 1, Endothelial monocyte-activating polypeptide II, Multisynthase complex auxiliary component p43
Detection Method:
Sandwich
Range:
125-8,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
