The Human AlphaAFP (Alpha-Fetoprotein) ELISA Kit is specifically designed for the precise measurement of alpha-fetoprotein levels in human serum, plasma, and cell culture supernatants. This ELISA kit guarantees exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Alpha-fetoprotein is a key biomarker associated with various medical conditions, including liver cancer, fetal development abnormalities, and liver diseases.
By detecting and quantifying alpha-fetoprotein levels, researchers can gain valuable insights into these conditions and potentially identify new treatment strategies.Overall, the Human AlphaAFP ELISA Kit is a valuable tool for studying alpha-fetoprotein and its implications in different disease states, ultimately contributing to advancements in medical research and clinical diagnosis.
Product Name:
Human alphaFP (Alpha-Fetoprotein) ELISA Kit
SKU:
HUES01357
Target:
Human alphaFP (Alpha-Fetoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human αFP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human αFP. You can calculate the concentration of Human αFP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
95-109
85-97
Average (%)
91
101
92
1:4
Range (%)
103-115
82-93
94-108
Average (%)
108
88
99
1:8
Range (%)
100-115
81-93
96-112
Average (%)
106
87
102
1:16
Range (%)
99-113
85-97
95-113
Average (%)
105
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
94-108
99
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.5
15.2
39.5
5.6
15.6
39.3
Standard deviation
0.3
0.8
1.5
0.3
0.9
2.2
C V (%)
5.45
5.26
3.8
5.36
5.77
5.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human αFP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human αFP in samples. No significant cross-reactivity or interference between Human αFP and analogues was observed.
