The Human Alpha2-AP (Alpha2-Antiplasmin) ELISA Kit is specifically designed for the accurate detection of alpha2-antiplasmin levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Alpha2-antiplasmin is a critical protein that plays a key role in hemostasis by inhibiting fibrinolysis. Dysregulation of alpha2-antiplasmin has been linked to various medical conditions such as thrombosis, bleeding disorders, and liver diseases, making it a crucial biomarker for studying these diseases and potential therapeutic interventions.
Overall, the Human Alpha2-AP ELISA Kit offers researchers a valuable tool for accurately measuring alpha2-antiplasmin levels in human samples, advancing our understanding of hemostasis-related disorders and potentially guiding the development of new treatment strategies.
Product Name:
Human alpha2-AP (alpha2-Antiplasmin) ELISA Kit
SKU:
HUES02090
Target:
Human alpha2-AP (alpha2-Antiplasmin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
7.50 ng/mL
Detection range:
12.50-800 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human α2-AP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human α2-AP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human α2-AP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human α2-AP. You can calculate the concentration of Human α2-AP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
94-105
93-106
Average (%)
102
99
100
1:4
Range (%)
91-106
87-98
87-98
Average (%)
99
93
93
1:8
Range (%)
88-102
84-99
83-95
Average (%)
95
91
90
1:16
Range (%)
89-104
86-101
82-96
Average (%)
96
93
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.88
118.96
393.16
39.63
122.85
396.21
Standard deviation
2.07
5.92
16.0
2.22
6.15
13.87
C V (%)
5.06
4.98
4.07
5.6
5.01
3.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human α2-AP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human α2-AP in samples. No significant cross-reactivity or interference between Human α2-AP and analogues was observed.
