Human alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit
The Human Alpha-1-Acid Glycoprotein (AGP) ELISA Kit is specifically designed for the accurate and sensitive detection of alpha-1-acid glycoprotein levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results in a variety of research applications.Alpha-1-acid glycoprotein is an important acute phase protein that is involved in various physiological processes, including immune response regulation, drug binding, and inflammation modulation. Abnormal levels of AGP have been linked to certain diseases such as cancer, inflammatory disorders, and infectious diseases, making it a valuable biomarker for diagnostic and research purposes.
By using the Human Alpha-1-Acid Glycoprotein ELISA Kit, researchers can gain valuable insights into the role of AGP in health and disease, aiding in the development of potential therapeutic interventions and diagnostic tools. This kit is easy to use and provides accurate measurements, making it an invaluable tool for studying the complexities of alpha-1-acid glycoprotein in human biology.
Product Name:
Human alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit
SKU:
HUES01288
Target:
Human alpha1-AGP (Alpha-1-Acid Glycoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AGP1. You can calculate the concentration of Human AGP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
85-98
93-105
Average (%)
99
91
100
1:4
Range (%)
91-106
83-95
86-98
Average (%)
98
90
93
1:8
Range (%)
87-99
81-95
84-95
Average (%)
94
87
89
1:16
Range (%)
89-101
82-92
87-100
Average (%)
96
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
93-110
100
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.59
17.82
93.78
9.89
17.95
97.74
Standard deviation
0.54
0.94
3.55
0.52
0.93
4.54
C V (%)
5.63
5.27
3.79
5.26
5.18
4.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AGP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGP1 in samples. No significant cross-reactivity or interference between Human AGP1 and analogues was observed.
