Human Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit

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SKU:
HUDL00005
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02760
Sensitivity:
1.8ng/mL
Range:
4.687-300ng/mL
ELISA Type:
Sandwich
Synonyms:
AMBP,UTI,HCP,EDC1,HI30,IATIL,ITILC,ITI,ITIL,Alpha 1 Microglobulin/Bikunin Precursor,Growth-inhibiting protein 19,Uristatin,Uronic-Acid-Rich Protein,Trypstatin
Reactivity:
Human
Sample Type:
Serum, Plasma, Urine And Other Biological Fluids
Frequently bought together:

Description

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Human Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit

The Human Alpha-1-Microglobulin Bikunin Precursor (A1M) ELISA Kit is specifically designed for the accurate detection of A1M levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.A1M is an important protein involved in various physiological processes, including antioxidant and tissue protecting functions. It has been implicated in diseases such as kidney disorders, cardiovascular diseases, and preeclampsia, making it a valuable biomarker for studying these conditions and developing potential treatments.

With our Human A1M ELISA Kit, researchers can confidently analyze A1M levels in biological samples, providing valuable insights into disease mechanisms and potential therapeutic targets. Trust in our kit for accurate and efficient detection of A1M to advance your research efforts.

Product Name:Human Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit
Product Code:HUDL00005
Size:96 Assays
Target:a1M
Synonyms:AMBP, UTI, HCP, EDC1, HI30, IATIL, ITILC, ITI, ITIL, Alpha 1 Microglobulin/Bikunin Precursor, Growth-inhibiting protein 19, Uristatin, Uronic-Acid-Rich Protein, Trypstatin
Detection Method:Sandwich
Range:4.687-300ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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