Human Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) ELISA Kit

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SKU:
HUDL00149
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05186
Sensitivity:
36pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
BALP,BSAP,HOPS,AP-TNAP,TNSALP,Bone Alkaline Phosphatase,Alkaline Phosphatase,Tissue-Nonspecific Isozyme
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

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Human Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) ELISA Kit

The Human Alkaline Phosphatase (ALPL) ELISA Kit is specifically designed for the accurate detection of alkaline phosphatase levels in human liver, bone, and kidney samples. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.Alkaline phosphatase is an enzyme that plays a vital role in bone growth, liver function, and kidney health.

Abnormal levels of alkaline phosphatase can indicate various diseases and conditions, making it a valuable biomarker for studying these disorders and potential treatment options.The Human ALPL ELISA Kit is an essential tool for researchers looking to accurately measure alkaline phosphatase levels in human tissue samples, helping to further understand its role in health and disease.

Product Name:Human Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) ELISA Kit
Product Code:HUDL00149
Size:96 Assays
Target:ALPL
Synonyms:BALP, BSAP, HOPS, AP-TNAP, TNSALP, Bone Alkaline Phosphatase, Alkaline Phosphatase,Tissue-Nonspecific Isozyme
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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