Human Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit
The Human Alkaline Phosphatase Intestinal (ALPI) ELISA Kit is a cutting-edge tool for the precise measurement of ALPI levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing robust and consistent results, making it indispensable for various research endeavors.ALPI is an enzyme crucial for the hydrolysis of phosphate esters, playing a vital role in numerous biological processes. Dysfunction of ALPI has been linked to various diseases, including inflammatory bowel disease, liver disorders, and bone disorders.
Consequently, monitoring ALPI levels is essential for understanding these conditions and developing effective diagnostic and therapeutic strategies.With its advanced technology and reliability, the Human Alkaline Phosphatase Intestinal (ALPI) ELISA Kit is a valuable asset for researchers and clinicians alike seeking to unravel the complexities of ALPI biology and its implications in health and disease.
Product Name:
Human Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit
Product Code:
HUDL00148
Size:
96 Assays
Target:
ALPI
Synonyms:
CIP, IAP, Calf Intestinal Alkaline Phosphatase
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
