Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) CLIA Kit
The Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) CLIA Kit is a highly reliable and accurate tool for measuring ALCAM levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it ideal for various research applications.ALCAM is a critical cell adhesion molecule that plays a key role in mediating cell-cell interactions and promoting leukocyte adhesion. It is involved in various physiological processes, including immune responses, inflammatory reactions, and tissue regeneration. Dysregulation of ALCAM expression has been linked to various diseases, such as cancer, autoimmune disorders, and inflammatory conditions, highlighting its importance as a potential biomarker for disease diagnosis and prognosis.
The Human ALCAM CLIA Kit offered by Assay Genie provides researchers with a valuable tool for studying the role of ALCAM in health and disease, enabling them to explore its potential as a therapeutic target and diagnostic marker. With its easy-to-use format and reliable performance, this kit is a valuable asset for any laboratory conducting research in the field of cell adhesion and immune response.
Product Name:
Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) CLIA Kit
SKU:
HUES00222
Target:
Human ALCAM (Activated Leukocyte Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALCAM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ALCAM. You can calculate the concentration of Human ALCAM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
86-100
88-100
Average (%)
100
92
94
1:4
Range (%)
87-102
90-103
98-110
Average (%)
94
95
104
1:8
Range (%)
99-113
93-106
91-106
Average (%)
105
99
98
1:16
Range (%)
101-115
96-110
100-116
Average (%)
107
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.18
117.82
396.2
51.34
120.92
410.08
Standard deviation
5.82
13.02
40.61
6.48
13.71
33.91
C V (%)
12.08
11.05
10.25
12.62
11.34
8.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ALCAM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ALCAM in samples. No significant cross-reactivity or interference between Human ALCAM and analogues was observed.
