The Human AHR (Aryl Hydrocarbon Receptor) ELISA Kit is specifically designed to quantitatively measure levels of the Aryl Hydrocarbon Receptor in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The Aryl Hydrocarbon Receptor is a key transcription factor involved in mediating the effects of environmental toxins and endogenous ligands.
Its activation has been linked to various diseases including cancer, immune disorders, and developmental abnormalities. By measuring levels of the Aryl Hydrocarbon Receptor, researchers can gain valuable insights into the role this receptor plays in disease pathology and potentially identify new therapeutic targets.
Product Name:
Human AhR (Aryl Hydrocarbon Receptor) ELISA Kit
SKU:
HUES01705
Target:
Human AhR (Aryl Hydrocarbon Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AhR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AhR. You can calculate the concentration of Human AhR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
96-110
90-101
Average (%)
100
103
96
1:4
Range (%)
89-100
84-95
81-96
Average (%)
95
89
88
1:8
Range (%)
92-106
84-96
87-97
Average (%)
99
89
92
1:16
Range (%)
88-101
84-97
86-100
Average (%)
93
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
1.92
9.91
1.0
1.98
10.09
Standard deviation
0.06
0.08
0.33
0.05
0.09
0.32
C V (%)
5.66
4.17
3.33
5.0
4.55
3.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AhR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AhR in samples. No significant cross-reactivity or interference between Human AhR and analogues was observed.
