Human AHCY Recombinant Protein (RPPB1363)
- SKU:
- RPPB1363
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- AHCY
- Synonyms:
- EC 3311
- SAHH
- AdoHcyase
- S-adenosyl-L-homocysteine hydrolase
- Source:
- Sf9 Insect cells
- Uniprot:
- P23526
Description
| Product Name: | Human AHCY Recombinant Protein |
| Product Code: | RPPB1363 |
| Size: | 5µg |
| Species: | Human |
| Target: | AHCY |
| Synonyms: | EC 3.3.1.1, SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, AHCY, Adenosylhomocysteinase. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | AHCY protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADLMSDKLPY KVADIGLAAW GRKALDIAEN EMPGLMRMRE RYSASKPLKG ARIAGCLHMT VETAVLIETL VTLGAEVQWS SCNIFSTQDH AAAAIAKAGI PVYAWKGETD EEYLWCIEQT LYFKDGPLNM ILDDGGDLTN LIHTKYPQLL PGIRGISEET TTGVHNLYKM MANGILKVPA INVNDSVTKS KFDNLYGCRE SLIDGIKRAT DVMIAGKVAV VAGYGDVGKG CAQALRGFGA RVIITEIDPI NALQAAMEGY EVTTMDEACQ EGNIFVTTTG CIDIILGRHF EQMKDDAIVC NIGHFDVEID VKWLNENAVE KVNIKPQVDR YRLKNGRRII LLAEGRLVNL GCAMGHPSFV MSNSFTNQVM AQIELWTHPD KYPVGVHFLP KKLDEAVAEA HLGKLNVKLT KLTEKQAQYL GMSCDGPFKP DHYRYHHHHH H |
AHCY is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AHCY controls the intracellular S-adenosylhomocysteine (SAH) concentration that is crucial for transmethylation reactions. AHCY deficiency causes hypermethioninemia.
AHCY Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 441 amino acids (1-432 a.a.) and having a molecular mass of 48.8kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions). AHCY is fused to a 6 amino acids His-Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SAHH: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD). A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. Belongs to the adenosylhomocysteinase family. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - selenoamino acid; Amino Acid Metabolism - cysteine and methionine; EC 3.3.1.1; Hydrolase Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: neuron projection; cytoplasm; melanosome; cytosol; nucleus Molecular Function:identical protein binding; adenyl nucleotide binding; adenosylhomocysteinase activity Biological Process: methylation; circadian sleep/wake cycle; S-adenosylmethionine cycle; sulfur amino acid metabolic process; xenobiotic metabolic process; response to hypoxia; S-adenosylhomocysteine catabolic process; one-carbon compound metabolic process; chronic inflammatory response to antigenic stimulus; response to nutrient Disease: Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency |
| NCBI Summary: | S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009] |
| UniProt Code: | P23526 |
| NCBI GenInfo Identifier: | 20141702 |
| NCBI Gene ID: | 191 |
| NCBI Accession: | P23526.4 |
| UniProt Secondary Accession: | P23526,Q96A36, A8K307, B3KUN3, E1P5P2, F5H737, |
| UniProt Related Accession: | P23526 |
| Molecular Weight: | 432 |
| NCBI Full Name: | Adenosylhomocysteinase |
| NCBI Synonym Full Names: | adenosylhomocysteinase |
| NCBI Official Symbol: | AHCY |
| NCBI Official Synonym Symbols: | SAHH; adoHcyase |
| NCBI Protein Information: | adenosylhomocysteinase; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase |
| UniProt Protein Name: | Adenosylhomocysteinase |
| UniProt Synonym Protein Names: | S-adenosyl-L-homocysteine hydrolase |
| Protein Family: | Adenosylhomocysteinase |
| UniProt Gene Name: | AHCY |
| UniProt Entry Name: | SAHH_HUMAN |
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