The Human AGT (Angiotensinogen) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of angiotensinogen in human samples. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for serum, plasma, and cell culture supernatants.Angiotensinogen is a key factor in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance in the body. Dysregulation of this system has been linked to various cardiovascular and renal diseases, making angiotensinogen a valuable biomarker for understanding and treating these conditions.
With the Human AGT ELISA Kit, researchers can confidently study the role of angiotensinogen in disease development and progression, paving the way for potential therapeutic interventions. Order your kit today and unlock new insights into the complex mechanisms of the renin-angiotensin system.
Product Name:
Human AGT (Angiotensinogen) ELISA Kit
SKU:
HUES01529
Target:
Human AGT (Angiotensinogen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AGT. You can calculate the concentration of Human AGT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
86-97
95-107
Average (%)
92
92
101
1:4
Range (%)
94-104
80-93
81-95
Average (%)
99
87
87
1:8
Range (%)
88-103
85-99
82-95
Average (%)
94
92
88
1:16
Range (%)
86-100
84-95
83-97
Average (%)
92
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.18
13.08
46.09
4.68
14.24
45.85
Standard deviation
0.34
0.75
2.12
0.26
0.61
1.89
C V (%)
6.56
5.73
4.6
5.56
4.28
4.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AGT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGT in samples. No significant cross-reactivity or interference between Human AGT and analogues was observed.
