The Human AGRP (Agouti-Related Protein) CLIA Kit is a reliable and accurate tool for the detection of AGRP levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications. AGRP is a key protein involved in regulating appetite and energy balance, making it a valuable biomarker for studying obesity, metabolic disorders, and other related conditions.
By accurately measuring AGRP levels, researchers can gain insight into the mechanisms underlying these conditions and potentially develop new therapeutic strategies. With its advanced technology and user-friendly design, the Human AGRP CLIA Kit is a valuable resource for researchers seeking to explore the role of AGRP in human health and disease. Get accurate and reliable results with this innovative kit from Assay Genie.
Product Name:
Human AgRP (Agouti Related Protein) CLIA Kit
SKU:
HUES00005
Target:
Human AgRP (Agouti Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AgRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AgRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AgRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AgRP. You can calculate the concentration of Human AgRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
88-100
90-104
Average (%)
104
94
97
1:4
Range (%)
85-95
94-109
88-102
Average (%)
90
101
95
1:8
Range (%)
87-100
99-112
87-103
Average (%)
93
105
94
1:16
Range (%)
86-99
100-119
97-114
Average (%)
93
108
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
95-113
103
Cell culture media (n=5)
91-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.81
80.27
188.2
27.5
74.73
172.76
Standard deviation
3.33
8.7
17.52
3.55
6.72
14.08
C V (%)
12.9
10.84
9.31
12.91
8.99
8.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AgRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AgRP in samples. No significant cross-reactivity or interference between Human AgRP and analogues was observed.
