Human AGR2 (Anterior Gradient Protein 2) ELISA Kit
The Human AGR2 (Anterior Gradient Protein 2) ELISA Kit is specifically designed to accurately measure AGR2 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a wide range of research applications.AGR2 is a key protein involved in various cellular functions, including protein folding and tumor progression.
Its dysregulation has been implicated in diseases such as cancer, inflammatory disorders, and neurodegenerative conditions, highlighting the importance of studying AGR2 levels. By utilizing this ELISA kit, researchers can gain valuable insights into the role of AGR2 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human AGR2 (Anterior Gradient Protein 2) ELISA Kit
SKU:
HUES01527
Target:
Human AGR2 (Anterior Gradient Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AGR2. You can calculate the concentration of Human AGR2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
93-106
87-101
Average (%)
105
99
93
1:4
Range (%)
92-104
85-98
81-93
Average (%)
98
91
87
1:8
Range (%)
89-101
82-95
82-93
Average (%)
95
87
87
1:16
Range (%)
93-108
85-98
84-98
Average (%)
99
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.53
4.95
0.48
1.64
4.79
Standard deviation
0.03
0.08
0.22
0.03
0.08
0.23
C V (%)
6.25
5.23
4.44
6.25
4.88
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AGR2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGR2 in samples. No significant cross-reactivity or interference between Human AGR2 and analogues was observed.
