Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). Initial studies investigating the role of RAGE in renal dysfunction focused on diabetes, neurodegenerative disorders, and inflammatory responses. However, RAGE also has roles in the pathogenesis of renal disorders that are not associated with diabetes, such as obesity-related glomerulopathy, doxorubicin-induced nephropathy, hypertensive nephropathy, lupus nephritis, renal amyloidosis, and ischemic renal injuries. RAGE represents an important factor in innate immunity against pathogens, but it also interacts with endogenous ligands, resulting in chronic inflammation. RAGE signaling has been implicated in multiple human illnesses, including atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.
Product Name:
Human AGER/RAGE Recombinant Protein (RPES1130)
Product Code:
RPES1130
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Advanced Glycosylation End Product-Specific Receptor, Receptor for Advanced Glycosylation End Products, AGER, RAGE
Accession:
NP_001127
Sequence:
Met 1-Ala 344
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human AGER-His at 10 µg/mL (100 ?l/well) can bind biotinylated mouse His-S100A1 with a linear range of 15.6-250 ng/mL.3. Measured by its ability to bind biotinylated human S100A1 in functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human AGER (NP_001127) extracellular domain (Met1-Ala 344) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
35.5 kDa
AP Mol Mass:
47-53 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
