Human AGER (Advanced Glycosylation End Product Specific Receptor) ELISA Kit
The Human AGER (Advanced Glycosylation End Product-specific Receptor) ELISA Kit is a cutting-edge tool for the precise measurement of AGER levels in human biological samples. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it perfect for a variety of research studies.AGER is a key receptor that plays a vital role in recognizing and responding to advanced glycation end products, which are formed during the aging process and in various disease states.
By measuring AGER levels, researchers can gain valuable insights into the mechanisms underlying aging, diabetes, and inflammatory disorders. This ELISA kit is an indispensable resource for investigating the role of AGER in health and disease, paving the way for the development of targeted therapies and interventions.
Product Name:
Human AGER (Advanced Glycosylation End Product Specific Receptor) ELISA Kit
SKU:
HUES01525
Target:
Human AGER (Advanced Glycosylation End Product Specific Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGER. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AGER. You can calculate the concentration of Human AGER in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
95-112
88-103
Average (%)
98
102
95
1:4
Range (%)
97-112
83-94
96-109
Average (%)
104
89
101
1:8
Range (%)
101-114
85-95
94-105
Average (%)
108
90
99
1:16
Range (%)
98-112
88-101
95-110
Average (%)
105
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
265.2
685.1
1975.8
282.4
722.6
2137.8
Standard deviation
16.7
33.6
96.8
16.9
41.9
100.5
C V (%)
6.3
4.9
4.9
5.98
5.8
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AGER concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGER in samples. No significant cross-reactivity or interference between Human AGER and analogues was observed.
