Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit
The Human Acidic Fibroblast Growth Factor 1 (FGF1) ELISA Kit is specifically designed for the precise measurement of FGF1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results, making it the perfect choice for a variety of research purposes.FGF1 is a key growth factor that plays a crucial role in various biological processes, including cell growth, development, and wound healing.
Its dysregulation has been linked to several diseases such as cancer, diabetes, and cardiovascular disorders, highlighting its importance as a biomarker for disease detection and prognosis.By using the Human Acidic Fibroblast Growth Factor 1 (FGF1) ELISA Kit, researchers can gain valuable insights into the role of FGF1 in physiological and pathological conditions, paving the way for the development of innovative treatments and therapies.
Product Name:
Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit
SKU:
HUES01358
Target:
Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFGF/FGF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AFGF/FGF1. You can calculate the concentration of Human AFGF/FGF1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
87-101
93-108
Average (%)
99
93
99
1:4
Range (%)
96-110
85-98
97-112
Average (%)
102
90
103
1:8
Range (%)
96-108
82-96
98-110
Average (%)
101
88
104
1:16
Range (%)
95-111
83-97
96-110
Average (%)
102
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.6
246.2
965.3
92.5
240.6
1002.9
Standard deviation
5.5
12.1
37.6
5.9
13.0
34.1
C V (%)
5.58
4.91
3.9
6.38
5.4
3.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AFGF/FGF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed.
