The Human AF4/FMR2 Family Member 1 (AFF1) ELISA Kit is specially designed for the precise and accurate measurement of AFF1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for various research applications.AFF1, a member of the AF4/FMR2 family, is known to play a critical role in gene regulation and transcriptional activation.
Dysregulation of AFF1 has been implicated in various diseases such as leukemia and neurodevelopmental disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.With the Human AFF1 ELISA Kit, researchers can confidently measure AFF1 levels in human samples, advancing our understanding of disease mechanisms and paving the way for novel therapeutic interventions.
Product Name:
Human AF4/FMR2 Family, Member 1 (AFF1) ELISA Kit
Product Code:
HUDL00100
Size:
96 Assays
Target:
AFF1
Synonyms:
AF-4, AF4, AF4-MLL, MLL/AF4, MLLT2, PBM1, Myeloid/Lymphoid Or Mixed-Lineage Leukemia, Translocated To 2, Pre-B-Cell Monocytic Leukemia Partner 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
