Human ADRP (Adipose Differentiation Related Protein) ELISA Kit
The Human ADRP (Adipose Differentiation-Related Protein) ELISA Kit is specifically designed for the precise measurement of ADRP levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.ADRP is a key protein involved in adipogenesis and lipid metabolism, playing a crucial role in the regulation of lipid droplet formation within adipocytes.
Dysregulation of ADRP has been associated with metabolic disorders, obesity, and insulin resistance, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human ADRP ELISA Kit provides researchers with a reliable tool for investigating the role of ADRP in adipose tissue biology and metabolic disorders, offering insights into potential treatment strategies and personalized medicine approaches.
Product Name:
Human ADRP (Adipose Differentiation Related Protein) ELISA Kit
SKU:
HUES01513
Target:
Human ADRP (Adipose Differentiation Related Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADRP. You can calculate the concentration of Human ADRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
98-109
97-108
Average (%)
104
104
103
1:4
Range (%)
90-101
87-101
85-96
Average (%)
95
92
91
1:8
Range (%)
90-105
81-91
87-100
Average (%)
97
86
94
1:16
Range (%)
86-100
85-99
87-98
Average (%)
93
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.51
4.21
0.57
1.41
4.21
Standard deviation
0.03
0.07
0.22
0.03
0.08
0.14
C V (%)
5.66
4.64
5.23
5.26
5.67
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADRP in samples. No significant cross-reactivity or interference between Human ADRP and analogues was observed.
