GBP28,ApM1,AdipoQ,Acrp30,ACDC,APM1,C1Q And Collagen Domain Containing,Adipocyte Complement-Related Protein Of 30 KDa,Adipose Most Abundant Gene Transcript 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Human Adiponectin (ADP) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of adiponectin in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.Adiponectin is a key adipokine that plays a crucial role in regulating insulin sensitivity and metabolism.
Low levels of adiponectin have been associated with obesity, insulin resistance, and cardiovascular disease, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human Adiponectin ELISA Kit offers researchers a reliable and efficient way to study adiponectin levels and their impact on various health conditions.
Product Name:
Human Adiponectin (ADP) ELISA Kit
Product Code:
HUDL00090
Size:
96 Assays
Target:
ADP
Synonyms:
GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
