Human Adenylyl Cyclase Associated Protein 2 (CAP2) ELISA Kit
The Human Adenylyl Cyclase-Associated Protein 2 (CAP2) ELISA Kit is specifically designed for the sensitive detection of CAP2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it suitable for a variety of research applications.CAP2 is a key protein that plays a critical role in various cellular functions, including cell signaling and cytoskeletal organization. Dysregulation of CAP2 has been linked to diseases such as cancer, neurological disorders, and immune system dysfunction, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human CAP2 ELISA Kit, researchers can accurately measure CAP2 levels in human samples, providing valuable insights into its role in disease pathology and potential therapeutic strategies. Order now to advance your research in the field of CAP2 biology.
Product Name:
Human Adenylyl Cyclase Associated Protein 2 (CAP2) ELISA Kit
Product Code:
HUDL00422
Size:
96 Assays
Target:
CAP2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
