The Human ADA (Adenosine Deaminase) ELISA Kit is a versatile and reliable tool for the precise measurement of adenosine deaminase levels in human biological samples. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Adenosine deaminase is an enzyme involved in nucleic acid metabolism, playing a crucial role in the immune system and cellular function. Abnormal levels of adenosine deaminase have been linked to various diseases, including immunodeficiency disorders and certain types of cancer.
Therefore, this ELISA kit is essential for studying these conditions and developing potential diagnostic and therapeutic strategies.Overall, the Human ADA ELISA Kit offers researchers a valuable tool for investigating the role of adenosine deaminase in health and disease, with the precision and reliability needed for meaningful results.
Product Name:
Human ADA (Adenosine Deaminase) ELISA Kit
SKU:
HUES01499
Target:
Human ADA (Adenosine Deaminase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADA. You can calculate the concentration of Human ADA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
91-106
98-114
Average (%)
100
96
104
1:4
Range (%)
91-103
86-102
87-101
Average (%)
96
93
92
1:8
Range (%)
89-103
83-97
88-100
Average (%)
96
90
94
1:16
Range (%)
89-103
79-89
87-101
Average (%)
96
85
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.61
9.8
1.1
2.65
10.78
Standard deviation
0.07
0.11
0.3
0.07
0.11
0.5
C V (%)
6.54
4.21
3.06
6.36
4.15
4.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADA in samples. No significant cross-reactivity or interference between Human ADA and analogues was observed.
