The Human ACVB (Activin B) ELISA Kit is specifically developed for the precise measurement of Activin B levels in human biological samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for various research purposes.Activin B is a key regulatory protein that plays a vital role in various biological processes including cell differentiation, embryogenesis, and tissue regeneration. Dysregulation of Activin B has been linked to several health conditions such as cancer, inflammation, and metabolic disorders, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.
With its superior performance and reliability, the Human ACVB ELISA Kit is an essential tool for researchers and clinicians interested in investigating the role of Activin B in health and disease. Order your kit today from Assay Genie to advance your research endeavors.
Product Name:
Human ACVB (Activin B) ELISA Kit
SKU:
HUES02540
Target:
Human ACVB (Activin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACVB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACVB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACVB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACVB. You can calculate the concentration of Human ACVB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
92-108
99-113
Average (%)
94
100
104
1:4
Range (%)
89-104
83-95
89-99
Average (%)
97
88
94
1:8
Range (%)
92-103
85-96
84-94
Average (%)
97
90
88
1:16
Range (%)
91-105
79-91
82-94
Average (%)
97
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
96-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.34
99.71
484.0
52.29
96.13
493.46
Standard deviation
3.22
4.91
18.3
3.14
4.96
16.68
C V (%)
6.15
4.92
3.78
6.0
5.16
3.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ACVB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACVB in samples. No significant cross-reactivity or interference between Human ACVB and analogues was observed.
