The Human ACTN4 (Actinin Alpha 4) ELISA Kit is specially designed for the precise measurement of actinin alpha 4 levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for various research applications.Actinin alpha 4 is a key protein involved in cell adhesion, migration, and cytoskeletal organization. Abnormal expression of actinin alpha 4 has been associated with various diseases including cancer, kidney diseases, and cardiovascular disorders, highlighting its importance as a biomarker for disease progression and potential therapeutic targets.
Overall, the Human ACTN4 (Actinin Alpha 4) ELISA Kit provides researchers with a reliable and efficient method for studying the role of actinin alpha 4 in disease pathology and exploring its potential as a diagnostic marker or therapeutic target.
Product Name:
Human ACTN4 (Actinin Alpha 4) ELISA Kit
SKU:
HUES02638
Target:
Human ACTN4 (Actinin Alpha 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACTN4. You can calculate the concentration of Human ACTN4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
96-110
99-114
Average (%)
96
103
106
1:4
Range (%)
93-105
86-97
83-95
Average (%)
98
91
90
1:8
Range (%)
93-108
85-98
88-99
Average (%)
100
91
94
1:16
Range (%)
89-101
81-92
86-96
Average (%)
94
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
88-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.57
7.92
21.02
2.82
7.78
20.77
Standard deviation
0.17
0.41
0.78
0.15
0.36
1.04
C V (%)
6.61
5.18
3.71
5.32
4.63
5.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ACTN4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACTN4 in samples. No significant cross-reactivity or interference between Human ACTN4 and analogues was observed.
