Human Activity Dependent Neuroprotector Protein (ADNP) ELISA Kit
The Human ADNP (Activity-Dependent Neuroprotector Protein) ELISA Kit is a comprehensive tool for accurately measuring ADNP levels in human samples, including serum, plasma, and cell culture supernatants. This kit is renowned for its exceptional sensitivity and specificity, ensuring that researchers obtain reliable and consistent results for a wide array of research endeavors.ADNP is a critical protein known for its role in neuroprotection and neuronal development, making it a key player in various neurological disorders and conditions.
By studying ADNP levels, researchers can gain valuable insights into conditions such as Alzheimer's disease, autism spectrum disorders, and other neurodegenerative illnesses, potentially leading to the development of novel therapeutic interventions.Overall, the Human ADNP ELISA Kit offers a valuable tool for researchers looking to delve deeper into the intricate mechanisms of neuroprotection and neurological disorders, ultimately paving the way for innovative advancements in the field of neuroscience.
Product Name:
Human Activity Dependent Neuroprotector Protein (ADNP) ELISA Kit
Product Code:
HUDL00086
Size:
96 Assays
Target:
ADNP
Synonyms:
ADNP1, ADNP Homeobox 1, Activity-dependent neuroprotective protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
