Activin Receptor Type-2A is a protein that in humans is encoded by the ACVR2A gene. ACVR2A is an activin type 2 receptor. This gene encodes activin A type II receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins; composed of a ligand-binding extracellular domain with cysteine-rich region; a transmembrane domain; and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding; resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases.
Product Name:
Human Activin RIIA/ACVR2A Recombinant Protein (RPES1977)
Immobilized Human INHBC-His(Cat: PKSH032587) at 0.8µg/ml(100 ?l/well) can bind Human ACVR2A-Fc-6His. The ED50 of Human ACVR2A-Fc-6His is 6.73 ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Activin Receptor Type-2A is produced by our Mammalian expression system and the target gene encoding Ala20-Pro134 is expressed with a Fc; 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
41.2 kDa
AP Mol Mass:
36 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
