Human Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit

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SKU:
HUDL00034
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9BWD1
Sensitivity:
31.5pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
ACTL,Acetoacetyl Coenzyme A Thiolase,Acetyl-CoA transferase-like protein,Cytosolic acetoacetyl-CoA thiolase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Human Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit

The Human ACAT2 (Acetyl-Coenzyme A Acetyltransferase 2) ELISA Kit is specifically designed for the accurate and precise detection of ACAT2 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring trustworthy and reproducible results for a variety of research purposes.ACAT2 is a pivotal enzyme involved in lipid metabolism, particularly in the synthesis of cholesterol and fatty acids.

Dysregulation of ACAT2 has been linked to various metabolic disorders and cardiovascular diseases, making it a crucial biomarker for understanding these conditions and potentially developing novel therapeutic interventions.With its advanced technology and user-friendly design, the Human ACAT2 ELISA Kit is an indispensable tool for researchers aiming to unravel the complexities of lipid metabolism and its implications on human health.

Product Name:Human Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit
Product Code:HUDL00034
Size:96 Assays
Target:ACAT2
Synonyms:ACTL, Acetoacetyl Coenzyme A Thiolase, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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